The invention belongs to the technical field of genetic engineering and in particular relates to a targeted epigenome regulated and controlled hepatitis B virus (HBV)-resisting molecule HBx1C and application thereof. A brand-new protein molecule HBx1C gene, which has a targeted specific affinity interaction effect on an HBV gene and can be used for carrying out epigenetic methylation modificationon DNA (Deoxyribonucleic Acid) of the HBV gene, is designed according to bioinformatics, epigenetic inheritance engineering and an immunology principle; an adenovirus vector is introduced into cells,and a targeted and specific protein molecule aiming at an HBV can be efficiently expressed; the methylation chemical modification on the virus gene also can be generated. In vivo and vitro experimentsprove that the targeted epigenome regulated and controlled HBV-resisting molecule HBx1C has a function of efficiently inhibiting HBV surface antigen expression and a treatment effect of resisting theHBV is realized; the targeted epigenome regulated and controlled HBV-resisting molecule HBx1C can be used for preparing a medicine for treating hepatitis B in epigenetic genetic engineering and has awide application prospect.