The invention provides an aminoglycoside type derivative and a preparing method and application thereof. The aminoglycoside type derivative is a novel aminoglycoside type derivative. According to thederivative, the C2' amino position and the C6' amino position of etimicin are mainly modified in chemical structure to obtain two derivatives, namely the derivative modified by C6'-NH2 and the derivative modified by two target positions of C2'-NH2 and C6'-NH2; chemical semi-synthesis and in-vitro antibacterial activity study are conducted. A result shows that the derivatives have the advantage ofhaving high resistance to drug-resistance bacteria like methicillin-resistant staphylococcus aureus and strains of expression N6' aminoglycoside modifying enzymes. The compound is a compound or salt thereof, or pharmaceutically acceptable salt or prodrug thereof shown in the formula, wherein the formula is shown in the description.