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发明授权
CN107988405B 一种印第安纳沙门氏菌PCR检测试剂盒及其非诊断性检测方法 有权

一种印第安纳沙门氏菌PCR检测试剂盒及其非诊断性检测方法
摘要：
本发明公开了一种印第安纳沙门氏菌PCR检测试剂盒及其非诊断性检测方法。所述试剂盒包括10×PCR缓冲液、2.5U/μl Taq DNA聚合酶、10mM dNTPs、印第安纳沙门氏菌特异性PCR检测引物、阳性对照和阴性对照，所述阳性对照物为印第安纳沙门氏菌ATCC51959基因组DNA；所述阴性对照为灭菌双蒸水。本发明还公开了一种应用所述试剂盒检测印第安纳沙门氏菌的PCR方法。本发明方法具有快速、简单、特异性强、灵敏度高的优点，无需沙门氏菌诊断血清即可检测印第安纳沙门氏菌，相比传统的沙门氏菌血清学分型方法，在检测时间与检测成本方面具有较大优势，适合于批量检测。
摘要（英）：

The invention discloses a salmonella Indiana PCR (polymerase chain reaction) detection kit and a non-diagnosis detection method thereof. The kit comprises a 10*PRC buffer solution, 2.5U/mul of Taq DNA(deoxyribonucleic acid) polymerase, 10mM dNTPs (deoxyribonucleotide triphosphates), a specific PCR detection primer of salmonella Indiana, a positive reference substance and a negative reference substance, wherein the positive reference substance is DNA of salmonella Indiana ATCC51959 genome; the negative reference substance is sterilized double distilled water. The invention also discloses a PRCmethod using the kit to detect the salmonella Indiana. The method has the advantages that the operation is quick and simple, the specificity is strong, and the sensitivity is high; the salmonella Indiana can be detected without using salmonella diagnosis serum; compared with the traditional salmonella serological typing method, more advantages are realized in aspects of ssdetection time and detection cost, and the method is suitable of batched detection.

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