The invention relates to an atropine medicine slow-release eye drop which comprises a 0.001-10%(w/v) atropine medicine inclusion compound, wherein the atropine medicine inclusion compound is preparedfrom an atropine medicine and a host compound through an inclusion function. The invention further relates to a preparation method of the atropine medicine slow-release eye drop. The atropine medicineslow-release eye drop provided by the invention is high in main medicine stability, appropriate in concentration acting on partial human eyes at the moment of being applied to eyes, small in side effect on human eyes, and capable of effectively preventing or relieving juvenile myopia.