The invention relates to a preparation method of cis-jasmone. The preparation method of the cis-jasmone comprises the following steps: mixing a first prefabricated alkali with 3-alkoxy-1-pentyne, andallowing the mixture to react at a first preset temperature for a first predetermined time, so as to obtain 3-alkoxy-1,2-pentadiene; mixing the 3-alkoxy-1,2-pentadiene, 3-methylcyclopent-2-enone and afirst catalyst, and allowing the mixture to react at a second preset temperature for a second predetermined time, so as to obtain 3-methyl-2-(2-pentynyl)-cyclopent-2-enone; and adding the 3-methyl-2-(2-pentynyl)-cyclopent-2-enone and a first solvent into a hydrogenation reaction flask, and then adding a second catalyst into the hydrogenation reaction flask, performing gas-exchanging treatment onthe hydrogenation reaction flask, and allowing the mixture to react at a third preset temperature for a third predetermined time, so as to obtain the cis-jasmone. According to the preparation method of the cis-jasmone, the preparation process is simpler, and the process difficulty and preparation difficulty are low, so that large-scale production and application are facilitated.