The invention relates to research on cancer cell metastasis, and particularly discloses a method for detecting a proteome in rectal cancer. According to the method, highly invasive rectal cancer cellsHCT116-I8 are screened by utilizing Transwell, and a protein or a pathway relating to cell invasion capacity is found by performing quantitative protein identification by combining an SILAC technology. Dynamic expression level of the protein in cells with high invasion capacity can be analyzed by a proteomic technology from an overall perspective, and a protein expression profile can be detectedpanoramically. The changes of proteomes in the cells with different invasion capacity in the rectal cancer can be detected in a high-throughput, high-precision and high-resolution manner by utilizinga highly invasive intestinal cancer model and a technology of screening invasion related functional protein by proteomics, and a protein pathway relating to invasion and metastasis can be found. The occurrence mechanism of diseases, such as cancer, can be explored efficiently by utilizing the technology, and a medicament acting target with high reliability is provided.