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发明授权
CN107828673B 一种高效表达来源于雅致放射毛霉的羧肽酶Y的方法 有权

一种高效表达来源于雅致放射毛霉的羧肽酶Y的方法
摘要：
本发明公开了一种高效表达来源于雅致放射毛霉的羧肽酶Y的方法，属于生物工程领域。本发明通过构建表达重组质粒pPICZα‑procpy，电转化毕赤酵母GS115，通过不断提高抗生素的方法，筛选得到具有不同拷贝数的表达重组菌株，再往得到的重组菌株中转入来源于毕赤酵母的伴侣蛋白BIP，最终得到一株高效表达来源雅致放射毛霉的羧肽酶Y的表达重组菌株，诱导表达6天之后，表达量达到了525±20mg/L。
摘要（英）：

The invention discloses a method for high-efficiency expression of carboxypeptidase Y derived from actinomucor elegans and belongs to the field of bioengineering. By construction of expression recombinant plasmid pPICZalpha-procpy and electrotransformation of pichia yeast GS115 and through a method for continuously raising antibiotic, expression recombinant strains with different copy numbers arescreened out; then, chaperone BIP derived from pichia yeast is inoculated into the recombinant strain so as to finally obtain an expression recombinant strain for high-efficiency expression of carboxypeptidase Y derived from actinomucor elegans; and after 6 days of induced expression, expression level reaches 525 +/- 20 mg/L.

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