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发明授权
CN107488645B 一种热稳定性提高的脂肪酶TTL突变体TTL-Ser61Asn及其基因和应用 有权

一种热稳定性提高的脂肪酶TTL突变体TTL-Ser61Asn及其基因和应用
摘要：
本发明涉及基因工程领域，具体涉及一种热稳定性提高的脂肪酶TTL突变体TTL‑Ser61Asn及其基因和应用。所述脂肪酶TTL突变体TTL‑Ser61Asn的氨基酸序列如SEQ ID NO.4所示，突变体TTL‑Ser61Asn在80℃水浴5min后剩余酶活为23.4％，提高至亲本脂肪酶的1.29倍。
摘要（英）：

The invention specifically relates to a lipase TTL mutant TTL-Ser61Asn and lipase TTL mutant gene both with improved thermal stability, and application thereof, belonging to the field of gene engineering. The amino acid sequence of the lipase TTL mutant TTL-Ser61Asn is as shown in SEQ ID No. 4. The residual enzyme activity of the mutant TTL-Ser61Asn is 23.4% after treatment in a water bath with a temperature of 80 DEG C for 5 min, an increase of 1.29 times of the residual enzyme activity of parent lipase.

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