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CN107488619B 一株产石杉碱甲和石杉碱乙的短波单胞菌SXZ-N10菌株及用途 有权

一株产石杉碱甲和石杉碱乙的短波单胞菌SXZ-N10菌株及用途
摘要：
本发明提供了一株产石杉碱甲和石杉碱乙的单胞菌SXZ‑N10菌株，该菌株可产石杉碱甲和石杉碱乙。该菌株被命名为SXZ‑N10，属一种单胞菌，该菌株保藏于中国典型培养物保藏中心，地址：中国，武汉，武汉大学；邮编430072，保藏日期为2017年9月14日，保藏编号为：CCTCC NO：M2017512；该菌株的16S rDNA序列如SEQ ID NO.1所示。上述单胞菌SXZ‑N10菌株能够用于发酵制备石杉碱甲和石杉碱乙。微生物发酵条件包括：发酵培养基组成：酵母粉8g/L，蛋白胨5g/L，氯化钠5g/L，葡萄糖10g/L，pH 6.0；培养条件为：27℃，200rpm，发酵培养7天。
摘要（英）：

The invention provides a monad SXZ-N10 strain capable of producing hperzine A and hperzine B. The strain is capable of producing the hperzine A and the hperzine B. The strain is name as SXZ-N10, and belongs to monad; the strain is preserved in China Center for Type Culture Collection (CCTCC), and the address of the CCTCC is Wuhan University, Wuhan, China; the postal code is 430072, the preservation date is September 14, 2017, and the preservation number is CCTCC No: M2017512; a 16S rDNA sequence of the strain is as shown in SEQ ID No.1. The monad SXZ-N10 strain can be used for preparing the hperzine A and the hperzine B by means of fermentation. The microbial fermentation conditions are as follows: a fermentation culture medium is prepared from 8g/L of yeast powder, 5g/L of peptone, 5g/L of sodium chloride and 10g/L of glucose, and has the pH value of 6.0; the culture conditions are 27 DEG C and 200rpm; fermentation culture is carried out for 7 days.

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