The invention discloses a jitter sequence generation method and apparatus capable of improving halftone image layers. The method comprises the following steps that: a first initial differentiation sequence and a second initial differentiation sequence are generated correspondingly according to a jitter space matrix; sequence elements in the first initial differentiation sequence which are larger than a preset threshold are initialized to be zero, and sequence elements in the second initial differentiation sequence which are smaller than or equal to the preset threshold are initialized to be zero, and therefore, a first jitter sequence and a second jitter sequence can be generated; and the flag element of a first flag sequence and the flag element of a second flag sequence are determined separately according to the first jitter sequence, the second flag sequence and a preset formula. According to the jitter sequence generation method and apparatus capable of improving the halftone image layers of the present invention, when matrix elements are outputted according to a sequence; the outputted matrix elements are uniform in geometric positions; and the difference values of the element values of the matrix elements and the element values of adjacent matrix elements are large, and therefore, special harmful print textures can be avoided.