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发明公开
CN107429243A 改良型β-呋喃果糖苷酶 无效 - 撤回

改良型β-呋喃果糖苷酶
摘要：
本发明提供能够抑制蔗果四糖的生成、高效生成蔗果三糖的改良型β-呋喃果糖苷酶。一种改良型β-呋喃果糖苷酶，其含有下述(a)或(b)的氨基酸序列：(a)对于序列号2所示的氨基酸序列导入了以下的i)和/或ii)的氨基酸变异的氨基酸序列，i)将N末端起第85位的甘氨酸(G)置换为甘氨酸(G)以外的蛋白组成氨基酸的氨基酸变异，ii)将N末端起第310位的组氨酸(H)置换为赖氨酸(K)、精氨酸(R)或酪氨酸(Y)的氨基酸变异；(b)含有在(a)中缺失、置换、插入或附加除了导入有氨基酸变异的氨基酸以外的一个或多个氨基酸的氨基酸序列且具有β-呋喃果糖苷酶活性的氨基酸序列。
摘要（英）：

To provide an improved beta-fructofuranosidase which is capable of efficiently producing kestose while inhibiting the production of nystose. This improved beta-fructofuranosidase comprises either: an amino acid sequence (a) obtained by introducing, into the amino acid sequence represented by SEQ ID NO: 2, an amino acid mutation i) in which the 85th glycine (G) from the N-terminal is substituted for a protein-constituting amino acid other than glycine (G), and/or an amino acid mutation ii) in which the 310th histidine (H) from the N-terminal is substituted for lysine (K), arginine (R), or tyrosine (Y); or an amino acid sequence (b) which exhibits beta-fructofuranosidase activity, and which comprises an amino acid sequence obtained by deleting, substituting, inserting, or adding one or more amino acids in (a) other than the amino acid into which the amino acid mutation has been introduced.

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