The invention discloses an antiseptic treatment method of a mold. The method comprises steps as follows: step 1, water, oil dirt, dust, pollutants, iron rust and oxidation scale on the surface of the mold are cleared away; step 2, the mold treated in the step 1 is washed with water; step 3, the mold washed with the water is dried; step 4, the mold is subjected to acid pickling in a single acid solution with the PH value smaller than or equal to 6 at the temperature of 80-90 DEG C for 30-60 s; step 5, surface carburization treatment is performed; step 6, an antiseptic liquid is sprayed or brushed on the surface of the mold, and an antiseptic layer is formed; step 7, the mold with the antiseptic layer is put in a baking oven at the temperature of 100-120 DEG C to be baked; step 8, the mold is taken out, and antiseptic treatment of the mold is completed. By means of the provided antiseptic treatment method of the mold, effective antiseptic treatment can be performed on the mold surface, the service life of the mold is prolonged, and the product quality is improved.