The invention relates to the field of peptide products, in particular to a production method of a yolk polypeptide product. Eggs are cooked, yolk is obtained and subjected to crushing and supercritical extraction for separation of oil, after residues are dealcoholized, low-temperature drying and dispersion are performed, a glutamine solution of 600-700 times is uniformly sprayed on a dispersed material, the dispersed material is inoculated with mucor for fermentation until a layer of fine fluff grows on the surface of the dispersed material, the dispersed material and water are uniformly mixed, high-speed centrifugal treatment is performed, residues are removed, a supernatant is concentrated, the concentrated liquid is subjected to spray drying, and the yolk polypeptide product is obtained. The produced yolk polypeptide product has the advantages that the production process is simple, the equipment requirement is not high, the cost is low, the polypeptide yield is high and the purity is high, and the obtained polypeptide product has significant functions in promoting skeleton growth and keeping healthy functions of skeletons.