The invention discloses a method for analyzing the anti-oxidation capability of an oil-soluble anti-oxidant in emulsion, and belongs to the field of a method for measuring anti-oxidation activity of a substance. Oxidation of lipid in the emulsion is accelerated by diazoisobutyl cyanamide instead of azobis(isobutyramidine) dihydrochloride, free radicals formed by initiation are mainly positioned at the water-oil interface, and the oil-soluble antioxidant can exert effect. Lipid oxidation in the real oil-in-water emulsion generally occurs at the water-oil interface instead of water phase or oil phase, so the diazoisobutyl cyanamide initiator can reflect the anti-oxidation behavior of the antioxidant in the real oil-in-water emulsion well.