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发明公开
CN107219216A 一种化学发光免疫检测婴儿米粉中黄曲霉毒素B1的方法 无效 - 撤回

一种化学发光免疫检测婴儿米粉中黄曲霉毒素B1的方法
摘要：
一种化学发光免疫检测婴儿米粉中黄曲霉毒素B1的方法，包括以下步骤：(1)样品前处理：将婴儿米粉与水按照5％稀释为样品液，37℃保湿封闭，备用；(2)制备黄曲霉毒素B1抗原AFB1‑BSA；(3)吖啶酯标记的黄曲霉毒素抗原AFB1‑BSA‑AE的制备；(4)建立标准曲线；(5)加样进行免疫反应测定；(6)读值定量。本发明的所述方法具有检出限低，灵敏度高，且快速方便，成本低，非常适用于婴儿食品，如米粉等中的黄曲霉毒素B1的检测。
摘要（英）：

The invention provides a method for chemiluminescent immunodetection of aflatoxin B1 in baby rice flour. The method comprises the following steps: (1) pretreatment of a sample: diluting baby rice flour with water to obtain a sample solution with a concentration of 5% and hermetically maintaining moisture in the sample solution at 37 DEG C for subsequent usage; (2) preparing aflatoxin B1 antigen AFB1-BSA; (3) preparing acridinium ester-labeled aflatoxin B1 antigen AFB1-BSA-AE; (4) establishing a standard curve; (5) introducing the sample solution and carrying out immunoreaction determination; and (6) reading values and carrying out quantification. The method provided by the invention has the advantages of low detecting limit, high sensitivity, rapidness, convenience and low cost and is applicable to detection of aflatoxin B1 in baby food, such as rice flour.

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