The present invention discloses a non-pyrotechnic stage separation structure comprising a shape memory alloy connection, a heating plate and a collector. One end of the shape memory alloy connection is bent and deformed under the condition that the temperate is less than the phase transition temperature, the other end of the shape memory alloy connection penetrates a first hold section and a second hold section of an aircraft, the first hold section and the second hold section are fixedly connected by a locking piece, and the collector covers the locking piece. The non-pyrotechnic stage separation structure is initiated in domestic, can meet connection and separation requirements of spacecrafts, and is applied to small-sized separation devices of spacecrafts. The non-pyrotechnic stage separation structure can be connected with and separated from the hold sections through the memory alloy and the heating plate. The non-pyrotechnic stage separation structure is simple in structure, reusable, and simple and convenient to install.