The invention discloses a breeding method for an industrialized bottle cultivated oyster mushroom variety. The breeding method comprises the following steps: by taking a high-yield strain P99 and a wild strain NY-2 as parent strains, creating a batch of new oyster mushroom varieties through a mono-spore hybridization technology; circularly planting by adopting an industrialized bottle cultivated mushroom producing manner; and screening a good strain suitable for industrialized production: P14Y6, wherein the strain is black, has no stems, a thick mushroom cap and good toughness and can be transported for a long distance. The mushroom producing temperature is 12 DEG C-15 DEG C, the mushroom producing uniformity is 95% or more, the average yield of a single bottle is 180g, the weight of culture materials of the single bottle is 250g and the maximum weight is 20 grams per bottle; the biological efficiency of one flush of the mushroom is 70% or more and the production period is 40 days; and no diseases and insect pests occur within a production period and year-round supply can be realized, so that the blank of special varieties of industrialized oyster mushrooms in China is made up.