The invention discloses a temperature sensitive polymer of coupled nucleic acids or proteins and use thereof, the synthesis method is as follows: 1) a to-be-coupled matter and a coupling agent are mixed evenly for reacting at 20-40 DEG C for 1-2 hours to obtain a coupling product; 2) the coupling product and a temperature sensitive polymer monomer are added into a dissolving solution, and mixed evenly to obtain a mixed solution, ammonium persulfate and tetramethylethylenediamine are added into the mixed solution for reacting for polymerization at the temperature below lower critical phase transition temperature for 2-10 hours to obtain a mixed system; and 3) the temperature sensitive polymer of the coupled nucleic acids or proteins with temperature sensitive properties can be obtained by heterogeneous separation and purification of the mixed system at the temperature higher than phase transition temperature. The temperature sensitive polymer can achieve homogeneous reaction and heterogeneous separation, can be used for biological detection and analysis, and can be used to complete efficient separation enrichment and analysis detection of different biological samples by use of specific bonding of a catch and a target only by changing biotin-labeled nucleic acid sequences or proteins.