The invention discloses a pumpkin stress-resistant gene CmNAC1 and belongs to the field of genetic engineering. A nucleotide sequence of the pumpkin stress-resistant gene is shown as SEQ ID NO.1. The invention further discloses an overexpression vector pHellsgate8-CmNAC1 established by the gene. The overexpression vector is used for converting arabidopsis thaliana, and an overexpression transformation plant is obtained. Results show that the phenotype of the whole plant greatly changes due to arabidopsis thaliana overexpression, leaf color becomes shallow, the plant changes short, the fertility, branching characteristic and the like changes, and meanwhile the drought, salt and low temperature stress resistance is also obviously improved. Therefore, the overexpression vector can be used for genetic improvement of crops.