The invention relates to a method for synthesizing 2-thienyl ethylamine by means of biological catalysis. The method comprises the steps of preparing glucose, disodium hydrogen phosphate and potassium dihydrogen phosphate into a water solution according to a certain proportion, sterilizing and cooling to obtain a bacillus licheniformis culture medium; activating the obtained bacillus licheniformis culture medium in a shaking table at the constant temperature according to a certain cell concentration; then, dissolving 2-nitro vinyl thiophene, adding the solution into the activated culture medium, carrying out a biological catalysis reaction, and then carrying out centrifugal separation; extracting supernatant by using ethyl acetate, washing, drying, and carrying out reduced pressure distillation to obtain the product 2-thienyl ethylamine. The method has the remarkable characteristics and progress of being simple in technology, high in synthetic yield, low in cost and less in environmental pollution.