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发明公开
CN106918664A 一种ASE‑HPLC法测定杜仲中松脂醇二葡萄糖苷的方法 无效 - 驳回

一种ASE‑HPLC法测定杜仲中松脂醇二葡萄糖苷的方法
摘要：
本发明公开了一种ASE‑HPLC法测定杜仲中松脂醇二葡萄糖苷的方法，属于化学检测领域。旨在提供一种故除杂步骤同药典，仅使用ASE替代提取过程以缩短前处理时间，同时保证了测定的准确率的ASE‑HPLC法测定杜仲中松脂醇二葡萄糖苷的方法，本发明采用ASE萃取方法萃取粉碎过的杜仲样品，其中，ASE萃取方法包括先在索氏提取器中采用三氯甲烷萃取，然后在快速溶剂萃取仪中用甲醇萃取，收集甲醇萃取液；再采用HPLC法检测甲醇萃取液中的松脂醇二葡萄糖苷含量。本发明用于测定杜仲中松脂醇二葡萄糖苷。
摘要（英）：

The invention discloses a method for determination of pinoresinol diglucoside in Eucommia ulmoides by an ASE-HPLC method and belongs to the field of chemical detection. The method utilizes an impurity removal process in the pharmacopeia, utilizes ASE to replace an extraction process, shortens pretreatment time and guarantees determination accuracy. A crushed sample of Eucommia ulmoides is extracted by an ASE extraction method. The ASE extraction method comprises extraction based on trichloromethane in a Soxhlet extractor, extraction based on methanol in a fast solvent extractor, methanol extract collection and detection of pinoresinol diglucoside content in the methanol extract based on a HPLC method. The method is used for determination of gluconol diglucoside in Eucommia ulmoides.

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