The invention provides preparation and application of an oxadiazole compound. The preparation method comprises the steps of: adding a first compound and a second compound into a container, and conducting reacting under the action of K2CO3 and THF (Tetrahydrofuran) to obtain a third compound, wherein the first compound is shown in the description, the second compound is shown in the description, the third compound is shown in the description, and R is selected from groups shown in the description; reacting the third compound under the action of N2H4-H2O and ethanol to obtain a fourth compound, wherein the fourth compound is shown in the description; reacting the fourth compound with a fifth compound under the action of the THF and triethylamine to obtain a sixth compound, wherein the fifth compound is shown in the description, and the sixth compound is shown in the description; reacting the sixth compound under the action of benzene sulfonyl chloride, potassium carbonate and dichloromethane to obtain a seventh compound, wherein the seventh compound is shown in the description; and reacting the seventh compound with an eighth compound under the action of CuI and Pd(PPh3)2Cl2 to obtain a ninth compound, wherein the eighth compound is shown in the description, and the ninth compound is a first target compound shown in the description. The compound prepared by the method has the effects of suppressing tyrosine kinase and treating leukemia.