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发明授权
CN106841079B 一种测定富含还原糖的蛋白样品中蛋白质含量的方法 有权 转让

一种测定富含还原糖的蛋白样品中蛋白质含量的方法
摘要：
本发明涉及一种测定富含还原糖的蛋白样品中蛋白质含量的方法。本发明先用DNS法测出富含还原糖的蛋白样品中还原糖的浓度，再通过还原糖与BCA试剂反应的等比关系计算出在利用BCA试剂测定富含还原糖的蛋白样品中蛋白质含量时扣除还原糖的贡献值，最后利用蛋白质与BCA试剂反应的等比关系计算出蛋白质的准确含量。本发明所述方法能排除富含还原糖的蛋白样品中还原糖的干扰，快速、精确的测定出富含还原糖的蛋白样品中蛋白质的含量。
摘要（英）：

The invention relates to a method for measuring the protein content in a protein sample rich in reducing sugar. According to the method, firstly, the concentration of the reducing sugar in the protein sample rich in the reducing sugar is measured by a DNS method; the contribution value of the reducing sugar is deducted when a BCA (bicinchoninic acid) reagent is used for measuring the protein content in the protein sample rich in the reducing sugar through the geometric proportion relationship of the reducing sugar and BCA reagent reaction; finally, the accurate content of the protein is calculated through the geometric proportion relationship of the reducing sugar and BCA reagent reaction. The method has the advantages that the interference of the reducing sugar in the protein sample rich in the reducing sugar can be eliminated; the protein content in the protein sample rich in the reducing sugar can be fast and precisely measured.

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