The invention discloses an anti-collision device of an AGV. The anti-collision device comprises a lifting plate, a laser barrier detector and a main controller; the lifting plate capable of moving up and down is arranged in the tail portion of the AGV; the laser barrier detector is arranged on the lifting plate; and the main controller is connected with the laser barrier detector. When a barrier is detected by the laser barrier detector, a signal is transmitted to the main controller, and the main controller stops the AGV according to a preset program. According to the anti-collision device of the AGV, a planar area that can be scanned by the laser barrier detector forms a 3D scanning scope with continuous up-down movement of the lifting plate, and a barrier suspended in the air can be scanned by the laser detector. Thus, the AGV is avoided from colliding with the barrier in the backward movement process.