The invention discloses a method for detecting the virus disease resistance of a leaf structure and root hairs. The method comprises the following steps: tearing tomato leaves with hands, carrying out tissue culture on a root, and observing the leaf surface structure and the root hairs through an electron microscope: in the middle growth stage of a plant, collecting a tender leaf of a normal leaf, tearing the tender leaf with the hands, observing an epidermis cell and the thickness of the leaf and villus and pores on the leaf; dipping tetrapanax papyriferus with alcohol with the concentration of 100 percent, adding the leaf in the middle of the tetrapanax papyriferus, cutting the leaf with two blades, selecting a cut leaf between the two blades, and observing the leaf through the electron microscope; disinfecting a seed, carrying out culture medium germination test on the seed, and carrying out electron microscope observation in different stages in the growing process to observe the quantity of the root hairs. According to the method, through the observation of the epidermis cell structure, the quantity of the villus and the quantity of the pores on the leaf, the quantity of the root hairs and the thickness of the leaf, an important basis can be supplied to judgment whether the plant is disease-resistant or not; an important basis is supplied to disease-resistant breeding in a tomato field; an effect is intuitive and simple, and the investment and the cost are low.