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发明公开
CN106754826A 活性提高的α‑淀粉酶AmyL突变体及其编码基因和应用 有权 转让

活性提高的α‑淀粉酶AmyL突变体及其编码基因和应用
摘要：
本发明涉及基因工程领域，具体涉及活性提高的α‑淀粉酶AmyL突变体及其编码基因和应用。突变体的氨基酸序列如SEQ ID NO.2所示。本发明的经过改良的α‑淀粉酶菌株在180小时的发酵酶活达到36900U/mL，比改造前的α‑淀粉酶生产菌提高41.5％。因此，本发明的突变α‑淀粉酶BsaAmy6及重组工程菌，大大降低了发酵生产成本，为其进一步工业化应用奠定基础。
摘要（英）：

The invention relates to the field of genetic engineering, and in particular to an alpha-AmyL (Amylase) mutant with increased activity and a coding gene and application thereof. An amino acid sequence of the mutant is as shown in SEQ ID NO. 2. The fermentation enzyme activity of an improved alpha-AmyL strain is up to 36900 U/mL, and compared with an alpha-AmyL producing strain before improvement, the fermentation enzyme activity is increased by 41.5 percent. According to mutant BsaAmy6 (Bacillus Salsus Alpha-Amylase 6) and recombinant engineering bacterium, the fermentation production cost is greatly reduced, and a foundation is laid for further industrial application.

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