The invention discloses a fruit and vegetable crisp puncture device with a bionic structure, and solves the problems of long drying time and poor production quality existing during drying and processing of conventional fruit and vegetable crisps. A pressing and lifting mechanism comprises a supporting frame, wherein an end cover is connected to the upper end of the supporting frame; a cylindrical sleeve is arranged in the supporting frame; an ejector rod is arranged in the cylindrical sleeve; a guide sleeve is arranged on the ejector rod; a spring sleeves the ejector rod; a handle is connected to the top end of the ejector rod; a puncture pressing head assembly comprises a pressing head connecting plate; a plurality of puncture pressing heads are connected to the lower end surface of the pressing head connecting plate; the puncture pressing heads are distributed in the form of the leaf vein nodes of deciduous shrub ligustrum vicaryi; each puncture pressing head adopts the shape of the tail stinger of apis cerana; a separating baffle above the base is connected to the base; and guide through holes are formed in the base and the separating baffle. According to the fruit and vegetable crisp puncture device disclosed by the invention, a moisture migration channel is constructed between the internal tissues of fruit and vegetable crisps, so that the drying efficiency of the fruit and vegetable crisps is improved, and the quality grade of products is improved.