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CN106676195A 一种用于丝状支原体山羊亚种的荧光定量PCR检测引物 有权

一种用于丝状支原体山羊亚种的荧光定量PCR检测引物
Abstract:
本发明提供一种用于丝状支原体山羊亚种的荧光定量PCR检测引物，所述引物序列为上游引物：5’‑ TGTTGTTGGTGGGTCTGCTT‑3’，下游引物：5’‑ ACTTTATCAGCCGCCATTTGA‑3’。本发明根据GenBank登录的FQ377874.1的MMLC_1770基因序列，设计特异性引物，于国内外首先建立丝状支原体山羊亚种的SYBR Green Ⅰ实时荧光定量PCR方法，该方法特异性好、灵敏度高、重复性好，可用于检测临床样品中丝状支原体山羊亚种的含量。
Abstract(English):

The invention provides a fluorescent quantitative PCR detection primer for mycoplasma mycoides subsp. capri. The primer has the following sequences: sense primer: 5'-TGTTGTTGGTGGGTCTGCTT-3', and anti-sense primer: 5'-ACTTTATCAGCCGCCATTTGA-3'. Based on the MMLC_1770 gene sequence of FQ377874.1 in GenBank entry, the invention designs the specific primer, and establishes the SYBR Green I real-time fluorescent quantitative PCR method for mycoplasma mycoides subsp. capri for the first time at home and abroad. The method has good specificity, high sensitivity and good repeatability, and can be used for detecting the content of mycoplasma mycoides subsp. capri in a clinical sample.

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