The present invention relates to an apparatus for cell fixation culture, wherein the apparatus comprises a microfluidic chip and a microdroplet fixation member, the microfluidic chip is provided with a solution injection channel and a droplet conveying channel, the solution injection channel is communicated to the droplet conveying channel, one end of the solution injection channel is provided with a solution injection port, the other end is communicated to the droplet conveying channel, one end of the droplet conveying channel is provided with an oil phase carrier liquid input port, the other end is provided with a droplet discharging port, and the microdroplet fixation member is arranged on the bottom surface or top surface of the droplet conveying channel. According to the present invention, the apparatus is suitable for suspension culture, adherent cell culture and microbial film culture, and is easy to observe and quantify, and by introducing the culture liquid or biochemical reagent, the long-term culture, testing and dynamic observation of cells can be achieved.