The invention relates to the technical field of foods, and especially relates to sucrose-free healthy candies prepared by natural plant fermentation. The sucrose-free healthy candies prepared by the natural plant fermentation comprises the following ingredients in parts by mass: 4-9 parts of L-arabinose, 1-6 parts of fructose, 1-3 parts of xylose, 0-2 parts of glucose, 1-3 parts of fruit and vegetable powder, and 0.2-3 parts of water. A preparation method of the sucrose-free healthy candies prepared by natural plant fermentation comprises the following steps: preparing corn-bran enzymatic hydrolysate and jerusalem artichoke juice; culturing strains; carrying out fermentation culturing; carrying out centrifugal filtration, decolorization and decompressed concentration so as to prepare healthy syrup; and then, adding the water and the fruit and vegetable powder into the syrup, carrying out filtrating, carrying out concentrating, adding auxiliary materials, carrying out cooling, carrying out shaping, carrying out sterilization, carrying out cooling and packaging, so that the sucrose-free healthy candies prepared by natural plant fermentation are prepared. The prepared sucrose-free healthy candies prepared by natural plant fermentation are suitable for sucrose-banned people, as well as people who are fond of sweets but afraid of the influence on the health. The required raw materials are wide in sources and low in cost, as well as easy to be controlled in physicochemical conditions, short in production circles, stable in product qualities, high in yields and convenient for industrialized production; moreover, the raw materials are free of season control.