The invention discloses a film pushing device for making a monolayer cell. The film pushing device comprises a substrate, of which both sides are provided with guide rail strips; a pushing piece, which can flatly pave cell fluid on the substrate by means of glidingly moving on the guide rail strips; a film pushing clip, which is connected with the pushing film; a first hinge component, which comprises a first hinge installing base and a first hinge, and connected with the film pushing clip through a fixing hole; a second hinge component, which comprises a second hinge installing base and a second hinge, and is connected with the first hinge component; the pushing directions of the first hinge and the second hinge are relatively perpendicular to each other in terms of space. By using the automatic fine tuning technology of a film pushing gesture on two degrees of freedom, the rapid continuous monomer spreading of 1 billion cells is realized; there is no superposition of multilayer cells. The film pushing device can be matched with an imaging device, so as to quickly find out the rare cells and lesion cells; therefore, the film pushing device is significant to the early clinical detection of the infectious disease, tumor and cancer.