The invention relates to a sheep peroxide redox enzyme 6 double antibody sandwich ELISA kit based on a BSaBA signal amplifying system, and belongs to the field of biological detection. The sheep peroxide redox enzyme 6 double antibody sandwich ELISA kit comprises a sheep-resistant Prdx6 rabbit polyclonal antibody pre-coated ELISA plate, a sheep Prdx6 recombinant protein standard substance, a diluent, a 20 * PBS-T concentrated scrubbing solution, a monoclonal antibody for detection, GaMIgG for biotin labeling, SaBA composite detection liquid, a 1g/L p-nitrophenyl phosphate PNPP substrate developing solution, a stop buffer, 10 * red cell lysate concentration storage liquid and glass beads with the diameters of 425 to 600 microns; the prepared sheep Prdx6 recombinant protein is used as immunogen to prepare the specificity sheep-resistant Prdx6 rabbit polyclonal antibody and is coated on the ELISA plate as a capturing antibody, the sheep Prdx6 recombinant protein is used as the standard substance, the specificity sheep-resistant Prdx6 rabbit polyclonal antibody is used as a detection antibody, and the BSaBA detection signal amplifying system is used for assembling a sheep Prdx6 content determination kit. The kit can be used for Prdx6 content determination in sheep white blood cell, serum, plasma and other tissue samples, and the sensitivity reaches a pg level.