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CN106243204A 一种毒蝇鹅膏免疫调节蛋白Fip-amu1及其制备方法和应用 无效 - 驳回

一种毒蝇鹅膏免疫调节蛋白Fip-amu1及其制备方法和应用
摘要：
本发明涉及一种毒蝇鹅膏免疫调节蛋白Fip-amu1及其制备方法和应用，免疫调节蛋白的氨基酸序列如SEQ ID NO.1所示。制备方法包括：体中，得到重组载体；(2)将上述重组载体转化宿主细胞，得到重组菌株；(3)培养上述重组菌株，诱导重组毒蝇鹅膏免疫调节蛋白Fip-amu1的表达；(4)经NI柱纯化、透析分离获取。所述免疫调节蛋白用于制备保健食品。本发明通过体外重组得到的毒蝇鹅膏免疫调节蛋白Fip-amu1能够明显促进脾淋巴细胞增殖、促进肿瘤坏死因子TNF-α的表达等免疫调节作用，因此，在提高机体免疫力方面具有应用潜力。(1)将毒蝇鹅膏免疫调节蛋白的cDNA序列连入载
摘要（英）：

The invention relates to an Amanita muscaria immunomodulatory protein Fip-amu1, and a preparation method and application thereof. The amino acid sequence of the immunomodulatory protein is disclosed as SEQ ID NO.1. The preparation method comprises the following steps: (1) connecting the cDNA (complementary deoxyribonucleic acid) sequence of the Amanita muscaria immunomodulatory protein into a vector to obtain a recombinant vector; (2) transforming host cells by using the recombinant vector to obtain a recombinant strain; (3) culturing the recombinant strain, and inducing the expression of the recombinant Amanita muscaria immunomodulatory protein Fip-amu1; and (4) carrying out NI column purification and dialysis separation to obtain the Amanita muscaria immunomodulatory protein Fip-amu1. The immunomodulatory protein is used for preparing health food. The Amanita muscaria immunomodulatory protein Fip-amu1 obtained by in-vitro recombination can obviously promote spleen lymphocyte proliferation, promote the expression of the tumor necrosis factor TNF-alpha and have other immunoregulation actions, and therefore, has application potential in the aspect of organism immunity enhancement.

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