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发明公开
CN106222199A 改变细胞命运的手段 无效 - 撤回

改变细胞命运的手段
摘要：
本发明公开了一种改变细胞命运的方法。该方法包括使细胞过表达具有选自下列氨基酸序列之一的蛋白质：(a)SEQ ID NO：1、2所示的氨基酸序列；(b)SEQ ID NO：1、2所示的氨基酸序列的一部分；(c)与(a)和(b)中所示氨基酸序列具有至少80％，优选至少90％，更优选至少95％，进一步优选至少99％序列同一性的氨基酸序列。利用该方法能够使细胞过表达Mkk3或Mkk6蛋白，进而能够有效改变细胞染色质结构,促进多能性基因组蛋白乙酰化修饰，提高多能性基因的表达，从而能够有效地改变细胞命运尤其是促进体细胞的重编程。
摘要（英）：

The invention discloses a method for changing cell fate. According to the method, cells over-express protein selected from one of the following amino acid sequences: (a) an amino acid sequence as shown in SEQ ID NO: 1 and 2; (b) a part of the amino acid sequence as shown in SEQ ID NO: 1 and 2; (c) an amino acid sequence having at least 80% homology of the amino acid shown in (a) and (b), preferably, the homology is at least 90%, more preferably, the homology is at least 95%, and the most preferably, the homology is at least 99%. By using the method, the cells can over-express Mkk3 or Mkk6 protein, and then the cell chromatin structure can be effectively changed, acetylation of pluripotent genome protein is promoted, the expression of pluripotent genomes is improved, and the cell fate can be effectively changed, particularly, reprogramming of the cells is promoted.

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