The invention relates to research of a human azoospermia and severe oligozoospermia detection method, and provides a rapid detection method for researching human azoospermia and severe oligozoospermia. The method includes the steps: acquiring samples; treating the samples; performing diagnosis by the aid of a real-time PCR (real-time polymerase chain reaction) platform; performing ELISA (enzyme-linked immuno sorbent assay) detection by the aid of sample treating results. The invention further provides a kit using the method. The kit comprises RNAs (ribose nucleic acid) and tissue metabolites, wherein the RNAs are extracted after the samples are treated. The research method helps patients suffering from the human azoospermia and the severe oligozoospermia to be rapidly detected and diagnosed, and the kit can help the patients to rapidly find pathogenesis for treatment.