The invention relates to a tissue culture method of Pardancanda norrisii. The method comprises steps as follows: 1) flower buds, receptacles, nodes or tender leaves of Pardancanda norrisii are selected as explants, a callus induction medium is inoculated with the explants, and culture is performed until adventitious buds are differentiated; 2) the adventitious buds are transplanted in a subculture multiplying medium until seedlings are differentiated; 3) the seedlings are transplanted in a rooting medium, and Pardancanda norrisii seedlings are obtained after culture and rooting. With the tissue culture method, good traits of Pardancanda norrisii can be kept, large-scale rapid breeding can be realized, the callus formation rate is high in the culture process, the growth state is good, and the final survival seedling rate is high.