The invention discloses a graphene-nano copper composite material heat conduction film and a preparation method thereof. The heat conduction film is composited by copper nano particles and multilayer graphene, the diameter of the copper particles is nano grade, and the copper particles are uniformly distributed among the graphene layers and are used for filling the defect position of graphene, and are used for bonding the upper graphene layer and the lower graphene layer. The heat conduction film can keep the transverse high thermal conductivity characteristic, effectively increase the longitudinal thermal conductivity, and can be used for heat radiation of an electronic device.