The invention belongs to the technical field of nucleic acid amplification, and relates to a highly specific probe for real-time detection of NASBA (Nucleic Acid Sequence Based Amplification) products and a method for detecting the NASBA products. The highly specific probe comprises two adjacent nucleotide sequences, bases from the first position to the fourth position at the 3' end of each of the two adjacent nucleotide sequences are ribonucleotide bases, and the remaining bases are all deoxyribonucleotide bases. The highly specific probe is applied to detection of the NASBA products, has high sensitivity and specificity, and reduces detection time, thereby enabling the NASBA detection technology to be better applied to detection of nucleic acid of pathogenic microorganisms.