The invention provides a method for separating and purifying edoxaban by chromatography. The separation method is chromatography, and the chromatographic conditions are as follows: the filler is an amylose-tri(3,5-xylylcarbamate) bonded silica gel, and the mobile phase is selected from a mixed solution comprising a non-polar solvent and a polar solvent. By selecting the chromatographic conditions, the edoxaban and seven enantiomers and nonenantiomers thereof can be simultaneously separated, and thus, the method has the characteristics of high speed, high simpleness, high efficiency and the like.