The invention relates to an establishing method for hypoxia-resistant megalobrama amblycephala. The establishing method comprises the following steps that firstly, a hypoxia-resistant F1 flora is established, wherein the hypoxia-resistant F1 flora is bred through megalobrama amblycephala hypoxia stress; secondly, a hypoxia-resistant F2 flora is established, wherein an F2 family is established with megalobrama amblycephala of the hypoxia-resistant F1 flora serving as parents, hypoxia stress breeding is carried out in the first age stage of the F2 family, and normoxia breeding is carried out in the second age stage; thirdly, a hypoxia-resistant F3 flora is established, wherein the selfing F3 flora is established with megalobrama amblycephala of the hypoxia-resistant F2 flora serving as parents, or with hypoxia-resistant F2 female megalobrama amblycephala serving as female parents, and carp sperms subjected to ultraviolet inactivation are used for inducing and establishing megalobrama amblycephal subtracting and thelykaryon mitosis to develop the F3 flora. The establishing method has the advantages that megalobrama amblycephala of the new hypoxia-resistant variety is bred, the hypoxia-resistant ability is remarkably improved, the growth advantage is achieved, and the growth advantage character is stably inherited and accumulated.