The invention discloses a yellow wine making method inhibiting ethyl carbamate formation based on a target Dal80p. The method comprises the following steps: soaking rice; steaming rice and sprinkling water; adding wheat koji, a starter and water, and mixing uniformly; putting in a jar and making a nest; and performing saccharification, fermentation and aftertreatment, wherein the starter contains saccharomyces cerevisiae powder without DAL80 gene. In the invention, by adopting a transcription inhibition factor Dal80p as a control target and adopting the saccharomyces cerevisiae part without DAL80 or totally replacing traditional starter Chinese yeast for yellow wine making, the formation of ethyl carbamate can be effectively inhibited in the making process; and meanwhile, a few limitation factors exist, and the influence on the alcoholic strength, flavor and quality of the finished product of yellow wine is not obvious.