The invention discloses a preparation method of newcastle disease glucoprotein virus antigen and product thereof. According to the preparation method, the F gene and HN gene of the newcastle disease virus are optimized according to virus prevalent trend prediction, a newcastle disease virus glucoprotein antigen gene and an optimized antigen gene or series-connected optimized antigen genes are combined in a bombyx mori bioreactor for expression, and the expressed antigen or prepared recombinant virus can provide effective immune protection for animals. The invention also provides a preparation method of a newcastle disease virus antigen gene carrying vector. The preparation method comprises the following steps of: cloning the optimized newcastle disease virus antigen gene or series-connected optimized antigen genes into the baculovirus carrying vector controlled by a mammal promotor in a combined way; and recombining to obtain the gene carrying vector controlled by the mammal promotor. After the newcastle disease virus antigen gene carrying vector enters an animal body in an injection or oral administration mode, the newcastle disease virus antigen gene carrying vector can be used for efficiently carrying the antigen gene in the animal body and effectively defending the attack of newcastle disease virus.