知搜-全球专利检索

  1. 登录
  2. 注册
发明公开
CN105695438A 一种利用重组大肠杆菌高效生产转肽酶SortaseA的方法 无效 - 驳回

一种利用重组大肠杆菌高效生产转肽酶SortaseA的方法
摘要：
本发明公开了一种利用重组大肠杆菌高效生产转肽酶Sortase A的方法，属于基因工程领域。本发明成功实现了Sortase A在大肠杆菌中的高效生产，解决了目前已有报道中该酶表达水平较低的问题，在7L发酵罐中进行生产，所得Sortase A产量可达295.2mg/L，比优化前提高了58.5％；单位质量菌体酶活可达4845U/mg，比优化前提高了52.8％。本发明通过对重组菌株发酵生产条件的系统优化，为后续Sortase A的工业化生产奠定了基础。
摘要（英）：

The invention discloses a method for efficiently producing transpeptidase sortase A by using recombinant escherichia coli, and belongs to the field of gene engineering. Efficient production of the sortase A in the escherichia coli is successfully achieved, the problem of lower expression level of the enzyme reported at present is solved, production is performed in a 7 L fermentation tank, and the yield of the sortase A can reach 295.2 mg/L and is increased by 58.5% than the yield before optimization; the enzyme activity of unit mass of thalli can reach 4845 U/mg and is increased by 52.8% than the activity before optimization. By system optimization of recombinant strain fermentation production conditions, a foundation is laid for subsequent sortase A industrialized production.

0/0