The invention discloses a novel picoampere current source composed of MOSFET devices without electrodes at the source ends. The novel picoampere current source comprises the MOSFET devices of the same type. The drains of the MOSFET devices of the same type are connected together and connected to an output end O. Electrode substrates of the MOSFET devices are connected together and connected to the ground. By means of the novel picoampere current source, output and control of current of dozes of picoampere can be achieved, and the strict requirements of a biological chip for current amplitude and power consumption can be met; meanwhile, the novel picoampere current source can be prepared into a corresponding integrated circuit chip; the involved MOSFET devices and the circuit have quite high compatibility with a traditional CMOS integrated circuit process, and therefore no special process is needed, and manufacturing cost is low.