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CN105624031A 核酸扩增反应装置和核酸扩增方法 无效 - 撤回

核酸扩增反应装置和核酸扩增方法
摘要：
本发明提供一种即使反应时间短也能够得到足够的核酸扩增反应产物的核酸扩增反应装置和核酸扩增方法。核酸扩增反应装置包含：安装部，可安装填充有核酸扩增反应液和比重小于核酸扩增反应液且不与核酸扩增反应液混合的液体的核酸扩增反应容器；第1加热部，将所述核酸扩增反应容器的第1区域加热到第1温度；第2加热部，将所述核酸扩增反应容器的第2区域加热到第2温度；和驱动机构，对第1配置和第2配置进行切换，上述第1配置为第1区域在重力作用的方向位于第2区域的下方，上述第2配置为第2区域在重力作用的方向位于上述第1区域的下方，核酸扩增反应液的镁离子浓度为1.8mM～9.0mM。
摘要（英）：

The invention provides a nucleic acid amplification reaction apparatus and a nucleic acid amplification method, and even through reaction time is short, enough nucleic acid amplification reaction products can be obtained. The nucleic acid amplification reaction apparatus includes: a fitting section capable of fitting a nucleic acid amplification reaction vessel filled with a nucleic acid amplification reaction mixture and a liquid which has a specific gravity different from that of the nucleic acid amplification reaction mixture and is immiscible with the nucleic acid amplification reaction mixture; a first heating section which heats a first region of the nucleic acid amplification reaction vessel to a first temperature; a second heating section which heats a second region of the nucleic acid amplification reaction vessel to a second temperature; and a driving mechanism which switches over between a first arrangement in which the first region is located lower than the second region in the direction of the gravitational force and a second arrangement in which the second region is located lower than the first region in the direction of the gravitational force, wherein the concentration of magnesium ions in the nucleic acid amplification reaction mixture is 1.8 mM or more and 9.0 mM or less.

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