The invention discloses a chemical regeneration method of an oxidized form nicotinamide adenine dinucleotide NAD (P)+. The method comprises the following steps of under the condition of oxygen or air atmosphere, adding catalytic amount of fiber-bridged flavin, and oxidizing NAD (P) H to obtain the NAD (P) +. The chemical regeneration method provided by the invention has the following advantages that a catalyst of a regeneration cofactor is small organic molecule which does not contain precious metal and is cheap and easy to get; a regeneration system can regenerate NADH and NADPH; the regeneration system has wider pH range and temperature range, and is applicable to oxidation reaction of various oxidation-reduction enzyme catalysis relying on nicotinamide.