The invention discloses a medicated leaven thin-layer chromatography detecting method which comprises the following steps: (1) test solution preparation: taking herb powder to be detected, adding methanol for extraction, filtering, concentrating, and adding methanol to be the constant volume to be used as the test solution; (2) reference solution preparation: preparing the reference solution from a scopoletin comparison product or preparing the reference solution from the scopoletin comparison product and a negative control herb respectively; (3) thin-layer detection: conducting sample application on the test solution and the reference solution on a thin-layer plate, developing by using petroleum ether-ethyl acetate-formic acid as a developing solvent, inspecting and comparing, wherein the ratio of petroleum ether to ethyl acetate to formic acid is (5-7):(3.5-4.5):(0.05-0.15). According to the medicated leaven thin-layer chromatography detecting method, various components in the medicated leaven can be effectively separated, the stable and reliable index component scopoletin can be found, the quality of the medicated leaven herb can be accurately and reliably monitored, and effective guarantee is provided for clinical effects.