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CN105385778A 高通量检测花生AhFAD2B基因SNP基因分型的引物及方法 无效 - 驳回

高通量检测花生AhFAD2B基因SNP基因分型的引物及方法
摘要：
本发明涉及高通量检测花生AhFAD2B基因SNP基因分型的引物及方法。引物包括：特异引物，核苷酸序列如SEQ ID No.1和SEQ ID No.2所示；通用引物，核苷酸序列如SEQ ID No.3所示。本发明可以高通量检测鉴别AhFAD2B基因等位基因变异情况，一次检测样本量最高可达1536个，检测成本低，快速、准确，大大提高了花生育种选择的效率和准确性。
摘要（英）：

The invention relates to primers and a method for detecting SNP gene typing of AhFAD2B genes of peanuts in high throughput. The primers comprise the specific primer of which the nucleotide sequence is shown as SEQ ID No.1 and SEQ ID No.2 and a universal prime of which the nucleotide sequence is shown as SEQ ID No.3. According to the primers and method, the allelic variation condition of the AhFAD2B genes can be detected and identified in high throughput, the maximum sample quantity of one-time detection can reach 1536, the detection cost is low, rapidness and accuracy are achieved, and the efficiency and accuracy of peanut breeding selection are greatly improved.

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