The invention discloses a hydrocracking catalyst and preparation and application thereof. The hydrocracking catalyst contains a molecular sieve solid acid component. The molecular sieve solid acid component is a molecular sieve mixture containing MoBeta type zeolite and at least one type of Beta type zeolite selected from zeolite except MoBeta type zeolite, wherein the weight ratio of MoBeta type zeolite to at least one type of Beta type zeolite selected from zeolite except MoBeta type zeolite in the molecular sieve mixture is 9:1-1:9. Compared with the prior art, when the catalyst is used for a hydrocracking reaction, the conversion activity of arene in diesel oil and the selectivity of a ring-open product are obviously improved.