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发明公开
CN105274133A 一种改造尿素代谢调控途径降低酿酒酵母尿素积累的方法 无效 - 驳回

一种改造尿素代谢调控途径降低酿酒酵母尿素积累的方法
摘要：
本发明公开了一种改造尿素代谢调控途径降低酿酒酵母尿素积累的方法，属于微生物遗传和分子生物学领域。本发明方法是对尿素代谢途径相关激活因子的改造，具体是在消除Dal81p的泛素化调控位点的基础上，对Dal81pK69A,K918A和Dal82p进行过量表达。按照本发明方法对酿酒酵母尿素代谢途径相关激活因子进行遗传改造后，可以从根本上较少酿酒酵母发酵过程中尿素的积累，尿素积累量减少了55.7％。
摘要（英）：

The invention discloses a method for reducing saccharomyces cerevisiae urea accumulation by modifying a urea metabolism regulation approach and belongs to the field of microbial genetics and molecular biology. According to the method, related activating factors of a urea metabolic pathway are modified, and particularly, on the basis of eliminating ubiquitination regulatory sites of Dal81p, Dal81p, and Dal82p are subjected to overexpression. After the related activating factors of the saccharomyces cerevisiae urea metabolic pathway are subjected to genetic modification according to the method, urea accumulation during saccharomyces cerevisiae fermentation can be reduced fundamentally, and urea accumulation quantity is reduced by 55.7%.

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